MSnID Package for Handling MS/MS Identifications
نویسنده
چکیده
منابع مشابه
Optimization of mass spectrometry-compatible surfactants for shotgun proteomics.
An optimization and comparison of trypsin digestion strategies for peptide/protein identifications by microLC-MS/MS with or without MS compatible detergents in mixed organic-aqueous and aqueous systems was carried out in this study. We determine that adding MS-compatible detergents to proteolytic digestion protocols dramatically increases peptide and protein identifications in complex protein m...
متن کاملComparison of two Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Bacterial Speciation
Bacterial identification relies primarily on culture-based methodologies requiring 24h for isolation and an additional 24-48h for speciation. Matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) is an emerging technology newly applied to the problem of bacterial speciation. We evaluated two MALDI-TOF MS systems (MS) on 720 consecutively isolated bacterial ...
متن کاملComparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level.
Bacterial identification relies primarily on culture-based methodologies requiring 24 h for isolation and an additional 24 to 48 h for species identification. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is an emerging technology newly applied to the problem of bacterial species identification. We evaluated two MALDI-TOF MS systems with 720 consecu...
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Modern nano-HPLC systems are capable of extremely precise control of solvent gradients, allowing high-resolution separation of peptides. Most proteomics laboratories use a simple linear analytical gradient for nano-LC-MS/MS experiments, though recent evidence indicates that optimized non-linear gradients result in increased peptide and protein identifications from cell lysates. In concurrent wo...
متن کاملSpectral probabilities and generating functions of tandem mass spectra: a strike against decoy databases.
A key problem in computational proteomics is distinguishing between correct and false peptide identifications. We argue that evaluating the error rates of peptide identifications is not unlike computing generating functions in combinatorics. We show that the generating functions and their derivatives ( spectral energy and spectral probability) represent new features of tandem mass spectra that,...
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